Last edited by Kazrall
Wednesday, July 29, 2020 | History

6 edition of Plasmids for therapy and vaccination found in the catalog.

Plasmids for therapy and vaccination

  • 178 Want to read
  • 34 Currently reading

Published by Wiley-VCH in Weinheim, New York .
Written in English

    Subjects:
  • Plasmids.,
  • DNA vaccines.,
  • Gene therapy.,
  • Plasmids.,
  • Vaccines, DNA.,
  • Gene Therapy.

  • Edition Notes

    Includes bibliographical references and index.

    Statementedited by M. Schleef.
    ContributionsSchleef, M.
    Classifications
    LC ClassificationsQH452.6 .P566 2001
    The Physical Object
    Paginationxix, 287 p. :
    Number of Pages287
    ID Numbers
    Open LibraryOL3972064M
    ISBN 103527302697
    LC Control Number2001281025
    OCLC/WorldCa45487633

    Plasmid DNA has also been used to express specific antigens on cell membranes, stimulating and enhancing the immune system’s response and memory, and thereby offers the potential to generate a new, safer generation of vaccines 8. Plasmid-DNA-based delivery vectors are, however, less effective at transfecting cells than the viral vectors by: Plasmids are covalently closed, double stranded, DNA molecules which carry genetic information. These macromolecules have been used for gene therapy and DNA vaccination, and belong to a new class of medicinal agents that contain genetic by:

    Interest in producing large quantities of supercoiled plasmid DNA has recently increased as a result of the rapid evolution of gene therapy and DNA vaccines. Owing to the commercial interest in these approaches, the development of production and purification strategies for gene-therapy vectors has been performed in pharmaceutical companies within a confidential by:   Recent advances in chromatographic purification of plasmid DNA for gene therapy and DNA vaccines: A review. Abdulrahman A(1), Ghanem A(2). Author information: (1)Chirality Program, Faculty of Science and Technology, University of Canberra, Cited by: 4.

      Transfection of cells, ear skin and detection of expressed proteins by Western blot and ELISA. HEK cells (8 × 10 5) were transiently transfected with 2 μg plasmid DNA by Fugene 6 Reagent (Roche, Indianapolis, IN) or by gene gun bombardment at psi (3 shots with 1 μg plasmid).After 24 h of growth (37 °C, 10% CO2) in Dulbecco’s Modified Eagles Medium supplemented Cited by: Plasmids are extra pieces of genetic material found in many cells that usually confer a specific property to the cell. These properties include antibiotic resistance, toxin production, and many other features. Plasmids are used in genetic engineering to generate recombinant DNAs and as a mechanism to transfer genes between organisms.


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Plasmids for therapy and vaccination Download PDF EPUB FB2

The book describes modified vector systems based on plasmids, as well as the potency of genomic research and vector design by informatics. Using the example of fish vaccination, the application of DNA vaccination in veterinary health care is reviewed, followed by a detailed overview of plasmid production technology Plasmids for therapy and vaccination book an industrial scale.

The book describes modified vector systems based on plasmids, as well as the potency of genomic research and vector design by informatics.

Using the example of fish vaccination, the application of DNA vaccination in veterinary health care is reviewed, followed by a detailed overview of plasmid production technology on an industrial : Hardcover.

Plasmids for Therapy and Vaccination. (Editor) ISBN: July Wiley-Blackwell Pages. E-Book $ O-Book. About the Author. Budding entrepreneurs, owners of bricks-and-mortar businesses who want to take their businesses online. Table of contents. The Biology of Plasmids Structures of Plasmid DNA Genetic Vaccination Author: Martin Schleef.

Shareable Link. Use the link below to share a full-text version of this article with your friends and colleagues. Learn more. General properties of plasmids Plasmid‐encoded phenotypes The clinical importance of plasmids Plasmid cloning vectors The Biology of Plasmids - Plasmids for Therapy and Vaccination - Wiley Online LibraryCited by: Developments in gene therapy, cell therapy, and DNA vaccination require a pharmaceutical gene vector that, on one hand, fulfils the properties to express the encoded information-preferably at the.

Summary This chapter contains sections titled: Introduction Topological structures of plasmids Supercoiling of DNA DNA intercalating dyes Analysis of plasmid structures Conclusion Structures of Plasmid DNA - Plasmids for Therapy and Vaccination - Wiley Online LibraryCited by: Peter Mayrhofer, Martin Schleef and Wolfgang Jechlinger, Use of Minicircle Plasmids for Gene Therapy, Gene Therapy of Cancer, /_4, (), ().

Crossref Wolfgang Jechlinger, Optimization and delivery of plasmid DNA for vaccination, Expert Review of Vaccines, 5, 6, (), ().Cited by: 7. Plasmid DNA (pDNA) is the base for promising DNA vaccines and gene therapies against many infectious, acquired, and genetic diseases, including HIV-AIDS, Ebola, Malaria, and different types of cancer, enteric pathogens, and by:   Introduction.

Gene therapy approaches are of increasing interest, and >1, clinical trials have already been approved worldwide. 1 Since the discovery that plasmid DNA injection can be an efficient way to express a specific protein in vivo, 2 the so-called “nonviral” gene therapy field has been growing.

The production of plasmid DNA requires specific markers able to select plasmid Cited by: Plasmids for therapy and vaccination. [M Schleef;] -- This is the first book specializing in plasmids and their biomedical use, including all relevant aspects of production, applications, quality, and regulations.

Readers will discover clinical Your Web browser is not enabled for JavaScript. Plasmids for therapy and vaccination.

[M Schleef;] -- This book summarizes the background of plasmids, presenting their structures and a set of clinical applications to provide an overview of the wide range of preventive and therapeutic applications. The wide spread of infectious diseases have provoked the scientists to develop new types of vaccines.

Among the different types of vaccines, the recently discovered plasmid DNA vaccines, have gained tremendous attentions in the last few decades as a modern approach of by: 4.

Book Review: Plasmids for Therapy and Vaccination. Edited by Martin Schleef Book Review: Plasmids for Therapy and Vaccination. Edited by Martin Schleef Kistner, Otfried BOOKS to construct modified vector systems for times less is better than more ; some Plasmids for Therapy and Vaccina- tion.

Edited by Martin Schleef. Plasmids for Therapy and Vaccination This is the first book specializing in plasmids and their biomedical use, including all relevant aspects of production, applications, quality, and regulations.

Readers will discover clinical applications for the wide range of preventive and therapeutic applications using plasmid DNA. Structures of Plasmid DNA.

In book: Plasmids for Therapy and Vaccination, pp - 43 development and first clinical approaches to use DNA vectors in gene therapy, cell therapy and DNA. Buy Plasmids for Therapy and Vaccination by Schleef, Martin (ISBN: ) from Amazon's Book Store.

Everyday low prices and free delivery on eligible : Hardcover. Plasmid DNA [] and now mRNA [] vaccines have generated significant interest and efforts because of their potential as platform technologies that could be used for a variety of applications ranging from prophylaxis to therapy and from personalized medicine to global health can be quickly made with fairly generic manufacturing processes and can be constructed directly from the Cited by: The use of plasmid DNA for vaccination and therapy is a relatively novel technology, with advantages and limitations as with other gene transfer techniques.

This is the first book specializing in plasmids and their biomedical use, including all relevant aspects of production, applications, quality, and regulations. Readers will discover clinical applications for the wide range of preventive and therap. The demand for plasmid DNA has increased vastly in response to rapid advances in its use in gene therapy and vaccines.

These therapies are based on the same principle, i.e. the introduction of.Abstract: The demand for plasmid DNA has increased vastly in response to rapid advances in its use in gene therapy and vaccines. These therapies are based on the same principle, i.e. the introduction of nucleic acids in human/non-human cells receptor to restore, cancel, enhance or Cited by: Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications Guilherme N.M.

Ferreira, Gabriel A. Monteiro, Duarte M.F. Prazeres and Joaquim M.S. Cabral Interest in producing large quantities of supercoiled plasmid DNA has recently increased as a result of the rapid evolution of gene therapy and DNA vaccines.